The purpose of this study was to evaluate the contribution of SHISA3 promoter methylation to laryngeal squamous cell carcinoma (LSCC). SHISA3 promoter methylation status and expression were determined using methylation-specific polymerase chain reaction (MSP) and quantitative real-time PCR (qRT-PCR) in 93 paired LSCC and adjacent normal tissues, respectively. Furthermore, the regulatory function of the SHISA3 promoter fragment was analyzed using a luciferase reporter assay. The results reveal that there is a significant increase in SHISA3 methylation in LSCC tissues compared with corresponding nontumor tissues (P = 4.58E - 12). The qRT-PCR results show a significant association between SHISA3 methylation and expression in LSCC (P = 1.67E - 03). In addition, the area under the receiver operating characteristic curve was 0.91. Consequently, a log-rank test and multivariate Cox analysis suggest that SHISA3 promoter hypermethylation is a predictor of poor overall survival for LSCC (log-rank P = 0.024; HR = 2.71; 95% CI = 1.024-7.177; P = 0.047). The results indicate that SHISA3 promoter hypermethylation might increase the risk of LSCC through regulation of gene expression and is a potential diagnostic and prognostic biomarker for LSCC.
BioMed research international. 2017 Feb 16 [Epub]
Zhisen Shen, Chongchang Zhou, Jinyun Li, Dong Ye, Hongxia Deng, Bin Cao, Wenjuan Hao, Lexi Lin, Yuna Zhang
Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China., School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China., Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315040, China.